Journal of Research in Ecology
JRE
Ecology
http://ecologyresearch.info/
0
2319-1546
2319-1554
en
2017
10
29
5
2
online
1
fulltext
en
Influence of temperature, concentration and volume of serum on alternative complement pathway activity in European pond turtle
(Emys orbicularis)
Ecology
Short Communication
Little is known about complement system as a component of innate immunity of the ectothermic vertebrates such as turtles. Serum complement is a valuable tool in determining the health status of turtles. In this article, the activation of alternative complement pathway of European pond turtle (Emys orbicularis), using standard haemolytic assays was done. Effect of concentration, volume and temperature of serum complement of Emys orbicularis, on unsensitized rabbit red blood cell hemolysis was measured. Serum concentrations of 25% v/v produced 4.17±0.23mm, 50%, 5.05±0.057mm, and 100%, 6.25±0.5 mm hemolysis. 10μL volume of serum resulted in 4.9±0.05mm, 20μl, 5.4±0.52mm and 30μL 6.2±0.19mm hemolysis. Incubation of sera at 5-15°C produced 5.02±0.05mm, 25°C, 5.17±0.095mm and at 35°C 6±0.05mm hemolysis. In this research clear data about the significant effect of concentration, volume and temperature of serum complement on alternative complement pathway activity in turtles were presented. These data suggested that the increased innate immunity induced by high developmental concentration, volume and temperature strength rise the resistance of turtles to the outbreak of diseases.
Emys orbicularis, serum, complement, reptilian, innate immunity.
1221
1226
http://ecologyresearch.info/documents/EC0406.pdf
Azadeh
Yektaseresht
Yes
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Amin
Gholamhosseini
No
Department of Aquatic Animal Health and Diseases, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Ali
Janparvar
No
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran