Journal of Research in Ecology
JRE
Ecology
http://ecologyresearch.info/
0
2319-1546
2319-1554
en
2017
09
01
5
2
online
1
fulltext
en
The effect of mycorrhizal inoculation (Glomus mosseae) and brassinosteroid on the amount of antioxidants in Pimpinella anisum l. under cadmium stress
Ecology
Original Research
Heavy metals are important environmental pollutants and their toxicity is considered as a major problem, because of ecological, evolutionary, environmental and nutritional effects. Many studies have shown that plants inoculated with mycorrhizal fungi or the use of brassinosteroids increased plants resistance to heavy metals. In this study, the effect of mycorrhiza inoculation with Glomus mosseae and 24-epibrassinolid (10-6 µM) on Pimpinella anisum antioxidants against cadmium chloride stress (0, 100, 200 and 800 ppm) were investigated and compared in green house and hydroponic condition. The results showed that cadmium stress reduced shoot protein content, but amount of H2O2, flavonoid, flavonol and phenolic compound increased. The activity of catalase, superoxide dismutase, peroxidase, polyphenol oxidase increased significantly in cadmium treatments. When plants were pretreated with brassinosteroid and inoculated with mycorrhizal fungi and then treated with cadmium H2O2 have reduced some antioxidative parameters such as flavonoid and anthocyanin content, and activity of ascorbate peroxidase, peroxidase and polyphenol oxidase increased significantly. Therefore, it can be concluded that in this plant brassinosteroid application or mycorrhizal inoculation have positive effects on strengthening the antioxidant system in this plant against cadmium stress.
Glomus mosseae, brassinosteroid, cadmium chloride, Pimpinella anisum.
1033
1051
http://ecologyresearch.info/documents/EC0483.pdf
Sepideh
Hajbagheri
No
PhD Student, Azad University, Iran
Hosein
Abbaspour
No
Biology Department, Azad University, Iran
Shekoofeh
Enteshari
sh_enteshari@yahoo.com
Yes
Biology Department, Payam Noor University, Iran
Saeed
Habibollahi
No
Chemistry Department, Payam Noor University, Iran
Alireza
Iranbakhsh
No
Professor of Biology Department, Azad University, Iran